5 EASY FACTS ABOUT NAD+ AND ITS IMPACT ON AGING AND AGE-RELATED DISEASES DESCRIBED

Next, we taken care of cells with distinct concentrations of NAD+. When extra for 24 h at eighty µM in HeLa and four hundred µM in U2OS cells, NAD+ impaired the incorporation of EdU (Fig. 2a and Extended Facts Fig. 1k,l). Additionally, 16 µM of NAD+ was sufficient to lower fork speed by ~forty% in HeLa cells without the need of impacting